hROR1/4T1
CBPG0072
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| I. Background | |||
| 受体酪氨酸激酶样孤儿受体（Receptor Tyrosine Kinase-like orphan receptor，ROR）是 Ⅰ型受体酪氨酸激酶（receptor tyrosine kinase，RTK）家族中的成员，是一类膜受体，主要 包括 ROR1 和 ROR2。其中 ROR1 主要顺利获得参与细胞内与细胞间信号传导，实现调节细胞 代谢、增殖、凋亡、分化、迁移、转移等功能。ROR1 在正常组织中（除了原肠胚时期） 表达量较低，但 ROR1 在多种肿瘤细胞高度表达，包括乳腺癌、卵巢癌、肾癌、肺腺细胞 癌、胃癌等实体瘤细胞以及某些血液肿瘤细胞。 | |||
| II. Introduction | |||
| Host Cell: | 4T1 | ||
| Expressed gene: | hROR1 | ||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
| Freeze Medium: | 90% FBS+10% DMSO | ||
| Culture Medium: | RPMI-1640+10%FBS+2μg/ml Puromycin | ||
| Mycoplasma Testing: | Negative | ||
| Storage: | Liquid nitrogen | ||
| III. Description of Host Cell Line | |||
| Organism: | Mouse | ||
| Tissue: | Mammary gland | ||
| Morphology: | Epithelial | ||
| Growth Properties: | Adherent | ||
| IV. Representative Data | |||
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Figure 1. Recombinant hROR1/4T1 stably expressing ROR1. |
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| V. In VIVO Data | |||
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Index |
Content |
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Animal |
BALB/C, 7weeks, female |
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Implanted Position |
Subcutaneous (right lower flank region ) |
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Injected Cell Numbers |
3×10^5/0.1ml |
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Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. hROR1/4T1 Mean Body Weight ± SEM.
2. Tumor Size
Figure 3.hROR1/4T1 Mean Tumor Volume ± SEM.
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
